Coding

Part:BBa_K4388001:Design

Designed by: Venkata Kalyan Ghadiyaram   Group: iGEM22_KCL_UK   (2022-09-28)


Rhodotorula glutinis Tyrosine Ammonia-Lyase Mutant (RgTAL S9N/A11T/E518V)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2021
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence was codon-optimized for E.coli K12 strain, sequence had two PstI sites and one NotI site which were removed to allow for RFC10 compatibility.


Source

Zhou, S., Liu, P., Chen, J., Du, G., Li, H. et Zhou, J.. (2016). Characterization of mutants of a tyrosine ammonia-lyase from Rhodotorula glutinis. Applied microbiology and biotechnology, 100(24), 10443‑10452. doi:10.1007/s00253-016-7672-8

Mutations added to wild-type sequence from GenBank code: KF765779.1

References